离子色谱法测定水稻秸秆木质纤维素水解物单糖

Measurement of composition of monosaccharide in lignocelluloses of rice straw by ion chromatography

采用离子色谱法测定辐照加酶处理水稻秸秆木质纤维素转化的单糖组成。用γ射线对水稻秸秆进行预处理,再用水解酶进行水解,用20 mg/L叠氮化钠水溶液(防腐抑菌)稀释水解溶液,将溶液分别经过0.22μm尼龙滤膜和OnGuardⅡRP柱(2.5cc,经甲醇和水活化),弃去初始6 mL后,收集1 mL流出液,应用离子色谱法测定各单糖组成。结果表明,水稻秸秆木质纤维素水解物由木糖、葡萄糖、鼠李糖、甘露糖、葡萄糖醛酸等单糖组成。测定方法的精密度RSD≤1.38%,平均回收率达96.6%。

Ion chromatographic was used to determine the monosaccharide composition in rice straw with enzymes irradiation samples of lignocelluloses.First of all,the ordinary rice straw needs to be set under gamma radiation with 1 500 kGY intensity as pretreatment,hydrolyzed with hydrolase and then diluted with 20 mg/L solution of sodium azide（antimicrobial preservative）.Then,let the solution go through the 0.22 μm nylon membrane and OnGuard Ⅱ RP column（2.5 cc,by methanol and water） respectively.1 mL effluent can be collected after discarding the initial 6 mL solution.Finally,the composition of monosaccharide should be measured by ion chromatography.The result was that the lignocelluloses hydrolyzes of rice straw was composed of the xylose,glucose,rhamnose,mannose and glucuronic acid monosaccharide,etc.