鲁米诺-邻菲啰啉-过氧化氢化学发光体系检测饮用水中Pb(Ⅱ)

Determination of Pb(Ⅱ) in drinking water by luminol-1,10-phenanthroline-H_2O_2 chemiluminescence system

采用流动注射与化学发光法相结合,建立Pb(Ⅱ)催化过氧化氢氧化鲁米诺和邻菲啰琳的化学发光体系,用以检测饮用水中的Pb(Ⅱ)。通过考察鲁米诺溶液浓度、过氧化氢溶液浓度、邻菲啰啉浓度、铅液pH值及缓冲液pH值等单因素,选定最初条件,通过正交试验选定最佳条件。结果表明,Pb(Ⅱ)对鲁米诺-邻菲啰啉-H2O2体系的发光强度有明显增强作用,在选定的最佳条件下,当Pb(Ⅱ)质量浓度为1.0×10-7～1.0×10-5 g/mL时,Pb(Ⅱ)对鲁米诺-邻菲啰啉-H2O2体系发光强度的促进作用与Pb(Ⅱ)质量浓度呈现较好的线性关系,利用这一线性关系,可快速测定饮用水中Pb(Ⅱ)的浓度。

Catalytic reaction of luminol-phenanthroline-H2O2 chemiluminescence by Pb（Ⅱ） was tested by combining flowing-injection and chemiluminescence.Through the single factor experiment about the concentration of luminous,hydrogen peroxide,1,10-phenanthroline,the pH value of Pb solution and buffer solutions,the initial was selected and the best condition was designated through orthogonal test.The results showed that Pb（Ⅱ） enhanced the chemiluminescence intensity of luminol-1,10-phenanthroline-H2O2,and the stimulation of Pb（Ⅱ） had a positive correlation with its concentration in drinking water when its concentration ranged from 1.0×10-7 g/mL to 1.0 ×10-5 g/mL.It proved to be a rapid method to determine the sudden change of Pb（Ⅱ） in drinking water and to carry out the goalas an early alarming of emergency contamination of Pb（Ⅱ）.