Ki-67在GCB及non-GCB弥漫性大B细胞淋巴瘤中的表达及意义

Expression and significance of Ki-67 in germinal centre and non-germinal centre B-like diffuse large B-cell lymphomas

目的探讨Ki-67在GCB及non-GCB型弥漫性大B细胞淋巴瘤中的表达及意义。方法收集60例DLBCL及19例淋巴结反应性增生组织,并制作其组织芯片,采用免疫组化观测CD1O、bcl-6、MUM1蛋白的表达对DLBCL进行生发中心样B细胞(germinal center B cell-like,GCB)和非生发中心样B细胞(non-GCB)免疫表型分类,同时检测Ki-67蛋白的表达。结果 (1)60例DLBCL中CD1O、bcl-6、MUM1蛋白的阳性表达率分别为33.3%、45%、63.3%,GCB类型29例(48.3%),non-GCB类型31例(51.7%)。(2)Ki-67蛋白在淋巴结反应性增生(不包括生发中心)阳性率明显低于DLBCL(P<0.05)。(3)Ki-67蛋白的表达在DLBCL中GCB及non-GCB亚型中有显著性差异(P<0.05)。结论 Ki-67的表达可能与DLBCL免疫学分型有关,在non-GCB中,Ki-67高表达。

Objective To investigate the expression and significance of Ki-67 in germinal centre B-cell like（GCB）and non-germinal centre B-cell like（non-GCB） diffuse large B-cell lymphomas（DLBCL）.Methods A tissue chip cotaining 60 cases of DLBCL and 19 cases of reactive hyperplasia of lymph nodes was constructed,using CD10,BCL-6,MUM1,the cases of DLBCL were subtyped molecuiary into GCB and non-GCB groups and the expression of Ki-67 protein were studied.Results（1）60 cases in DLBCL BCL-6,CD10,MUM1 protein positive expression rate were 33.3%,45%,63.3% respectively,GCB type 29 patients（48.3%）,non-GCB type 31 cases（51.7%）.（2） Ki-67 protein in lymph node reactive hyperplasia（not including germinal centers） were significantly lower than DLBCL（P0.05）.（3） Ki-67 protein expression in DLBCL in GCB and non-GCB subtypes were significantly different（P0.05）.Conclusion Ki-67 expression may be related with the immunosubgroups of DLBCL.in non-GCB,Ki-67 high expression.