大肠癌及癌旁组织中端粒酶活性的检测及临床意义

Detection of Telomerase Activity in Colorectal Cancer andIts Adjacent Tissues

目的： 建立测定端粒酶活性的ＰＣＲ－ ＴＲＡＰ 方法并探讨其在大肠癌诊断中的意义。方法： 应用端粒酶活性的ＰＣＲ－ ＴＲＡＰ 方法，对４０ 例大肠癌及其癌旁组织进行了检测。结果：４０ 例大肠癌组织中有３７ 例端粒酶活性呈阳性， 阳性检出率为９１ ．７５ ％ ， 癌旁组织中有２ 例阳性， 阳性检出率为５ ．００ ％ ； 两者相比差异有极显著性（ Ｐ＜０ ．００１） ；４０ 例大肠癌均为腺细胞癌， 其中１１ 例伴随淋巴结转移的标本中１０ 例检测出活性， 而在２９ 例未伴随淋巴结转移的患者中，有２７ 例检测出端粒酶的活性，两者相比，差异无显著性。结论：端粒酶活性增高可能是致大肠组织癌变的重要因素；端粒酶活性的检出可作为大肠癌诊断的指标之一。

Objective: To evaluate the diagnostic value of telomerase activity in patients with colorectal cancer. Methods: Telomerase activity in colorectal cancer and its adjacent tissues of 40 patients were detected by using of PCR-TRAP method. Results: The positive rates of telomerase activity in the specimens of colorectal cancer and its adjacent tissues were 91.75%and 5.00%respectively (P< 0.001). Meanwhile, telomerase activity was not related with the differentiation type of the tumor and metastasis. Conclusion: It is suggested that PCR-TRAP is a valuable method for assaying activity of telomerase. Telomerase activity is related to colorectal cancer and it may be a diagnostic marker especially in early stage.