大肠良恶性上皮细胞AgNOR定量研究

Study on nucleolar organizer regions in bioptic samples of benign and malignant colonic mucosa

目的：对正常大肠、结肠炎、大肠腺瘤、腺瘤癌变、大肠癌及癌旁组织ＡｇＮＯＲ 进行定量研究，探讨其临床意义。方法：采用胶体银染技术对大肠良恶性上皮细胞中ＡｇＮＯＲ 染色，于显微镜下计数。良性疾病为炎症１０ 例，腺瘤１２ 例，恶性疾病为腺瘤癌变９ 例，腺癌１３ 例。正常对照８ 例。结果：正常、炎症及癌旁组织、腺瘤组织ＡｇＮＯＲ 计数低于癌组织（ Ｐ＜ ０－０１） ，腺瘤组织ＡｇＮＯＲ 计数高于正常、炎症及癌旁组织（ Ｐ＜ ０－０５） ，腺癌术后生存３ 年以上者与１ 年内复发死亡者癌组织ＡｇＮＯＲ 计数无差别。结论：ＡｇＮＯＲ计数有助于鉴别大肠良恶性疾病，对判断预后无价值，大肠腺瘤应及时切除。

Objective:To explore the clinical significance of the changes of argentaffin protein in nucleolar organizer regions(AgNOR) in normal, inflammatory mucosa,adenoma,colonic cancer,and surrounding mucosa of cancer .Methods:AgNOR was dyed by silver colloid and counting of AgNOR was done under microscopy.Benign disease included 10 cases of colonic inflammation and 12 cases of colonic adenoma.Malignant disease included 9 cases of cancer transformed by adenoma,and 13 cases of colonic adenocarcinoma.And 8 cases of normal colonic mucosa was used as control.Results:Counting of AgNOR in normal,inflammatory mucosa,surrounding mucosa of cancer and adenoma tissue was lower than that in cancer tissue(P<0 01).Quantity of AgNOR in adenoma tissue showed increase as compared with that in normal,inflammatory and surrounding mucosa of cancer(P<0 05).Counting of AgNOR was of no significant difference between the 3 year survivals and the deads within 6 month.Conclusions:The AgNOR count is helpful to identify malignant and benign colonic diseases,and doesnt appear to be a useful prognostic variable in colonic adenocarcinoma.Colonic adenoma should be resected in time.