大肠杆菌产β-内酰胺酶培养基条件

Studies on Medium Conditions of β-lactamase Production by Escherichia coli

对大肠杆菌(Escherichia coli)产β-内酰胺酶培养基条件进行优化研究,以提高β-内酰胺酶活力。研究了碳源、混合碳源配比、氮源、混合氮源配比、氯化钠、微量元素等因素对菌体干重及β-内酰胺酶活力的影响。确定菌种最佳培养时间为40h,最佳培养基组成为:葡萄糖16g/L、可溶性淀粉4g/L、蛋白胨20g/L、酵母浸粉10g/L、氯化钠6g/L、Zn2+浓度0.9 mg/L,β-内酰胺酶活力达2 648.95 nkat,比培养基优化前提高了18%。

The medium conditions of β-lactamase production by Escherichia coli were studied.The influence of different carbon,the proportion of mixed carbon sources,nitrogen sources,the proportion of mixed nitrogen sources,sodium chloride,trace element and other factors on dry cell weight and activity of β-lactamase was researched.The best time for cultivating bacteria is 40 h,the optimum medium composition for the 16 g/L glucose,soluble starch 4 g/L,Peptone 20 g/L,yeast powder Baptist 10 g/L,sodium chloride 6 g/L,Zn2＋ concentration 0.9 mg/L,broth,the production of β-lactamase come to 2 648.95 nkat,medium before optimization than 18 %.