摘要
采用超声波辅助热水提取法研究了苇蘑子实体多糖的提取工艺。正交试验所得苇蘑子实体多糖最佳超声提取条件为:提取温度30℃,提取时间15min,料液比1∶30(g/mL),超声功率200W。苇蘑子实体多糖含量采用传统的苯酚-硫酸法检测。在精密度试验中,RSD为0.283%,表明精密度较好。在稳定性试验中,RSD为0.374%,表明在0h～8h之间的稳定性较好。实验结果显示,该方法适用于苇蘑子实体多糖的提取及含量测定。该方法简便、准确、稳定、灵敏度高。测得苇蘑中多糖含量为21%。
Hot water extraction method assisted by ultrasonic was applied to extract Agaricus campestris polysaccharide from Agaricus campestris in this paper.The optimal extraction conditions were determined as follows： temperature of extraction 30 ℃,time of ultrasonic extraction 15 min,solid-liquid ratio 1∶30（g/mL） and the power 200 W.Phenol-sulphuric method was applied to determine Agaricus campestris polysaccharide.In the precision experiment,RSD value was 0.283 %,showing that the precision was better.In the stability experiment,RSD value was 0.374 %,showing that the stability was better in 0 h-8 h.This metod is easy,credibility,accuration and specificity.variation coefficient.In the condition,the extraction percentage of soluble polysaccharides in Agaricus campestris was 21 %.
出处
《食品研究与开发》
CAS
北大核心
2011年第3期38-41,共4页
Food Research and Development
基金
天津市科委科技计划项目"草腐生食用菌优良品种筛选及安全栽培技术研究"(合同编号:08ZCZDNC04600)
关键词
苇蘑
多糖
苯酚-硫酸比色法
超声提取
Agaricus campestris
polysaccharide
phenol-sulfuric acid assay
Ultrasonic extraction
