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中国明对虾CrusFc-2基因的重组表达及抗血清制备

Prokaryotic Expression and Antiserum Preparation of CrusFc-2 in Chinese Shrimp,Fenneropenaeus chinensis
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摘要 利用PCR和DNA重组技术克隆了中国明对虾CrusFc-2基因的成熟肽编码序列并构建了pET-DsbA-CrusFc-2原核表达载体,转化E.coliBL21(DE3)后,经异丙基--βD硫代半乳糖苷(IPTG)诱导得到了重组蛋白.重组蛋白经纯化后免疫新西兰大白兔制备了抗血清,Western Blot分析表明CrusFc-2蛋白主要存在于对虾血细胞中. The sequence coding mature peptide of CrusFc-2 was cloned from Chinese shrimp Fenneropenaeus chinensis,and a prokaryotic expression vector pET-DsbA-CrusFc-2 was constructed by PCR and recombinant DNA techniques.Fused protein was induced by IPTG in transformed E.coli BL21（DE3）.The New Zealand white rabbits were immunized with the purified recombinant protein DsbA-CrusFc-2 to prepare antiserum.The results of western blot indicated that CrusFc-2 mainly existed in hemocyte of shrimp.
作者 柳峰松 王晓菲 李婷 郑立 谢松
机构地区 河北大学生命科学学院
出处 《河北大学学报（自然科学版）》 CAS 北大核心 2011年第2期181-185,共5页 Journal of Hebei University(Natural Science Edition)
基金 教育部高等学校博士学科点专项科研基金资助项目(20102301120005) 河北省自然科学基金资助项目(C2011201027)
关键词 中国明对虾 CrusFc-2基因 原核表达 抗血清 Fenneropenaeus chinensis CrusFc-2 gene prokaryotic expression antiserum
分类号 Q78 [生物学—分子生物学]
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参考文献19

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河北大学学报（自然科学版）
2011年 第2期

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