血必净注射液对脓毒症大鼠肠TLR4和NF-κB的影响

Effects of Xuebijing Injection on the Expression of TLR4 and NF-κB in Intestinal Tissues in Septic Rats

目的:探讨血必净注射液对脓毒症大鼠肠的保护作用及机制。方法:Wistar大鼠80只,随机分成4组:正常对照组(N,n=8)、假手术组(S,n=8)、脓毒症组(C,n=32)、血必净治疗组(X,n=32),测定各组大鼠肠组织内TLR4和NF-κB表达,肠组织ICAM-1免疫组织化学染色及观察病理形态。结果:脓毒症组各时间点肠组织内TLR4、NF-κB和ICAM-1表达明显高于假手术组(P<0.01),血必净注射液可明显抑制这种变化(P<0.01)。结论:血必净注射液通过下调肠组织内TLR4、NF-κB和ICAM-1表达减轻脓毒症大鼠的肠损伤。

Objective：To study the protective effects of Xuebijing on acute intestinal injury in rats with sepsis. Methods：Wistar rats were randomly divided into 4 groups： normal control group （N, n=8）; sham operated control group （S, n=8）; sepsis induced by CLP group （C, n=32）; Xuebijing treatment group （X, n=32）. The toll like receptor 4（TLR4） and nuclear factor-κB （NF-κB） levels in intestinal tissues in each groups were measured. The immunohistochemistry was used to assess the expression of the ICAM-i on the intestinal tissues. The pathologic lesions of intestinal tissues were observed. Results：The levels ofTLR4, NF-κB and ICAM-1 in intestinal tissues in C groups are higher than those in S group （P〈0.01）. In X groups, the changes are ameliorated compared with C groups （P〈0.01）. Conclusions：Xuebijing can decrease the expression ofTLR4, NF-κB and ICAM-1 in intestinal tissues. Consequently intestinal injury is prevented.