摘要
目的:建立HPLC法测定天麻头痛片中阿魏酸含量。方法:采用Diamonsil C_(18)色谱柱,以乙腈-0.1%冰醋酸(20:80)为流动相,检测波长316nm。结果:阿魏酸在0.00961～0.07688μg线性关系良好,r=0.9996,平均回收率为96.9%(n=5),RSD为1.8%。结论:本法简便、准确、重复性好,可用于天麻头痛片中阿魏酸的含量测定。
Objective： To establish RP-HPLC for determining the content of ferullic acid in Tianmatoutong Tablet.Methods：An RPHPLC method was developed.The chromatographic conditions were as follow： Diamonsil C18 column and acetonitrile-0.1% glacial acetic acid （20：80） as mobile phase, detection wavelength at 316nm.Results：The recovery range of ferullic acid was 0.00961-0.07688ug, r=0.9996.The average recovery rate was 96.9% and RSD was 1.8%.Conclusions：The method is simple and accurate,with good repeatability, and can be used for determination of ferullic acid in Tianmatoutong Tablet.
出处
《中国医药导刊》
2011年第2期330-331,共2页
Chinese Journal of Medicinal Guide
