双波长薄层扫描法测定小儿肺热咳喘口服液麻黄碱的含量

Determination of Ephedrine in Xiaoerfeirekeruan Oral Solution by Dual Wavelength Thin-layer Scanning Chromatography

目的:建立双波长薄层扫描法测定小儿肺热咳喘口服液中麻黄碱含量的方法。方法:选用展开剂为氯仿-甲醇-氨试液(20:3.5:0.6),测定波长:波长λs=490nm,参比波长:λR=440nm,用双波长薄层反射锯齿扫描,SX=7,狭缝0.4nnm×0.4nm,灵敏度:X1,扫描速度:12mm/min。结果:麻黄碱平均加样回收率为99.2%,RSD为1.62%(n=6):在1.0～5.0μg范围内线性关系良好。结论:本测定方法简便、准确、重现性好,可用于测定小儿肺热咳喘口服液麻黄碱的含量。

Objective： To establish method for determing content of ephedrine in xiaoerfeirekeruan oral solution by dual wavelength thin-layer scanning chromatography.Methods：Developing agent：Chloroform-Methanol-Ammonia test solution（20：3.5：0.6）;determination wavelength：490nm with compared wavelength 44nm.Sawtooth wave by dual wavelength thin-layer scanning chromatography, SX=7, slit 0.4nm × 0.4nm; sensitivity：×1; scanning speed： 12mm/min.Resuits：Sample recovery was 99.2%,RSD was 1.62%（n=6）;with good linear relation among range 1.0-5.0lag. Conclusions：The method was simple, accurate with good repeatability, which could be made as the method for determing content of ephedrine in xiaoerfeirekeruan oral solution.