刺五加果实中黄酮类成分的分离与鉴定

Isolation and Identification of Chemical Constituents of Flavones from the Fruits of Acanthopanax Senticosus Harms

目的:比分离、鉴定刺五加果实提取物中的化学成分。方法:采用硅胶柱、ODS开放柱和制备液相等色谱方法分离,并通过NMR和MS等谱学技术确定结构。结果:从提取物中分离得到5个黄酮类化合物,分别鉴定为木犀草素(1)、木犀草素-4"-O-β-D-葡萄糖苷(2)、木犀草素-3'-O-β-D-葡萄糖苷(3)、术犀草素-7-O-β-D-葡萄糖苷(4),木犀草素-7,3'-O-β-D-二葡萄糖苷(5)。其中化合物2～5为首次从五加属植物中分离得到。结论:从提取物中分离得到5个黄酮类化合物,分别鉴定为术犀草素(1)、木犀草素-4'-O-β-D-葡萄糖苷(2)、术犀草素-3'-O-β-D-葡萄糖苷(3)、木犀草素-7-O-β-D-葡萄糖苷(4),木犀草素-7,3'-O-β-D-二葡萄糖苷(5)。

Objective： To investigate the chemical constituents of the extract from thefruits of Acanthopanax senticosns Harms.Methods： The chemical constituents were isolated by various isolation methods （silica gel, ODS and HPLC column chromatography） and their structures were elucidated by the analysis of spectral data（NMR and MS）. Results：Five flavones compounds were obtained and elucidated as following： lutcolin（1）luteolin-4＇-O-β- D-glucoside （2）, lutcolin-3＇-O-β-D-glucoside （3）, luteolin-7-O-β-D-glucoside （4）, luteolin-7,3＇-O-β-D-di-glucoside（5）.Compound 2-5 were isolated from the Acanthopanax genus for the first time. Conclusions：Results five flavones compounds were obtained and elucidated as following： luteolin（1）, luteolin- 4＇-O-β-D-glucoside（2）, luteolin-3＇-O-β-D-glucoside （3）, luteolin-7-O-β-D-glucoside （4）, luteolin-7,3＇-O-β-D-di-glucoside（5）.