乌阳补心胶囊质量标准研究

Study on quality standard for Wuyangbuxin Capsules

目的建立乌阳补心胶囊的质量标准控制方法。方法采用薄层色谱法对本品中的何首乌、甘草、黄芪、淫羊藿进行薄层鉴别;采用高效液相色谱法对本品中的2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷进行含量测定。结果在薄层色谱图中可检出何首乌、甘草、黄芪、淫羊藿;2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.116~2.32μg范围内呈良好的线性关系（r=0.999 8）,平均回收率为100.40%,RSD=0.64%。结论所建立的方法可靠、准确、专属性强,可有效控制乌阳补心胶囊的质量。

Objective To establish the quality standard for Wuyangbuxin Capsules.Methods The Polygonum multiflorum Thunb.,Glycyrrhiza uralensis Fisch.,Astragalus membranaceus（Fisch.）Bge.var.Mongholicus（Bge.）Hsiao,Epimedium brevicornum Maxim in Wuyangbuxin Capsules were identified by TLC;The content of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside was dentermined by HPLC.Results The Polygonum multiflorum,Glycyrrhiza uralensis,Astragalus membranaceus and Epimedium were detected in TLC chromatogram.The linear range of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside was 0.116 ～2.32 μg（r=0.999 8）,and the average recovery was 100.40% with RSD=0.64%（n=5）.Conclusion The method was reliable,accurate and specifie,and can effectively control the quality of Wuyangbuxin Capsules.