天津主要河流中耐高温蛭弧菌的分离及生物学特性

Purification of Bdellovibrio with High Temperature Resistance in Tianjin Rivers and Preliminarily Studies on Its Biological Characteristics

从天津4条主要河流（独流减河,南运河,北运河,子牙河）中分离出了耐高温的蛭弧菌并进行了纯化。通过采用宿主双层琼脂平板法对从河水中筛选出耐高温菌株并对其进行宿主范围、pH、温度等生物学特性的测定,结果表明：耐高温蛭弧菌在pH 7.0~7.5生长最好;对热致死的大肠杆菌,嗜水气单胞菌,荧光假单胞菌等3种细菌仍有裂解作用且出斑时间较裂解活菌早;寄生谱测定发现其可侵染绝大多数革兰氏阴性菌和部分革兰氏阳性菌,且为专性寄生。

Bdellovibrio which could grown at high temperature conditions was screened and purified in Tianjin four main rivers.The biological characteristics were tested by double deck agar plate,including its host spectra,pH resistance,and cultivation temperature.The results showed that the Bdellovibrio with high temperature resistance growed well at pH 7.0~7.5,and it could prey on the dead bacteria earlier compared with the living bacteria,such as Escherichia coli,Aeromonas hydrophila,Pseudomonas fluorescens,mass of gram negative bacteria and some gram positive bacteria could be preied on by the Bdellovibrio,which is an obligate parasite.