摘要
目的 了解重型乙型肝炎( 乙肝) 病人血清乙肝病毒(HBV)DNAC基因启动子(CP) 的变异。方法 对用聚合酶链反应(PCR) 法扩增的血清HBVDNA 直接测序。结果 7 例亚急性重型肝炎病人的HBV 分离株CP区分别有2~12 个替代变异,1 例病人有11bp 的碱基插入。CP变异主要发生于CP的第1 和第2 个AT丰富区,nt1 762 和nt1 764 的替代变异见于7 例亚急性重型肝炎病人的4 例中,是CP变异的热点,其中3 例HBeAg 阴性,说明和HBeAg 阴性表型相关。CP的第3 个AT丰富区、HBV逆转录起始位点(DR1) 和前C基因、前基因组转录起始位点未见变异。结论 重型肝炎病人的HBVCP区存在较多的变异,CP变异主要发生于和前C基因相关的第1 和第2 个AT丰富区,可能和HBeAg
Objective To detect hepatitis B virus core promoter (CP) mutations in patients with fulminant hepatitis.Methods Polymerase chain reaction amplified serum HBV DNA fragments were directly sequenced. Results There were 2 12 nucleotide substitutions in CP region in the 7 subacute fulminant hepatitis patients studied. An 11 bp nucleotides insertion was found in one patient. Mutations in CP were usually seen in the first and the second A T rich regions. The A to T mutation at nt 1 762 and G to A mutation at nt 1 764 were found in 4 cases, 3 of them were HBeAg negative. The third A T rich region was kept intact in all the 7 patients, so did the initial site of HBV replication (DR1) and the intial site of mRNA transcription (1 783/1784 or 1 790±1 for precore mRNA and 1818 for pregenome C/P mRNA).Conclusion CP mutations in patients with fulminant hepatitis are common, most of the CP variations occur in the first and the second A T rich regions, and these mutations may impede the transcription of precore mRNA and affect the expression of HBeAg.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1999年第3期251-254,共4页
Chinese Journal of Experimental and Clinical Virology