EB病毒转化人外周血单个核细胞作为HCV复制模型的初步研究

Establishment of hepatitis C virus reproductive model of human peripheral blood mononuclear cells transformed by Epstein Barr virus

目的 复制丙型肝炎病毒（ＨＣＶ） 感染的细胞模型。方法 用ＥＢ病毒感染其ＨＣＶ 阳性患者的外周血单个核细胞并使其转化，获得ＨＣＶ的外周永生化Ｂ细胞株。以后，每隔１ 个月应用套式逆转录聚合酶链反应（ＲＴＰＣＲ） 检测１ 次培养细胞和上清中的ＨＣＶＲＮＡ。结果 ＨＣＶ可以在传代细胞中持续存在１ 年，培养细胞中的ＨＣＶ负链和培养上清中的ＨＣＶ 则呈间断阳性。结论 ＨＣＶ 可以在该细胞株中较长时间存在。

Objective For the present, one of the crucial questions to study pathogenic mechanism of hepatitis C and its drug therapy is to seek a suitable cell model which may support the replication of hepatitis C virus(HCV). In this article, we made effort on the study of this aspect.Methods Using Epstein Barr virus(EBV), we transformed the peripheral blood mononuclear cells(PBMC) isolated from a patient diagnosed as chronic hepatitis C. HCV RNA from the cultured cells and the supernatants were analysed by reverse transcriptase polymerase chain reaction(RT PCR) at an intervals of one month.Results HCV positive strand RNA could be detected continually in the cultured cells for more than one year since the establishment of the transformed PBMC clone whereas the negative strand RNA were also observed in the cultured cells. Interestingly, the HCV positive strand RNA could be identified in supernatants intermittently.Conclusion HCV may exist in the cultured cell line for a longer period and reproduce in and secrete out of the cells. This will offer a new cell model for studying the molecular biology and immunology of HCV.