大鼠心脏移植与心肌细胞坏死

Cardiac allograft and myocardial necrosis in rats.

目的 探讨心肌细胞坏死在心脏移植中的发生和发展情况， 为心脏移植的抗排斥反应治疗和预防提供依据。方法 用供体脾细胞（ＳＰＣ） 和环磷酰胺（ＣＰ） 预处理移植受体， ＨＥ染色对移植心脏的炎细胞浸润和心肌细胞坏死进行分析。结果 经ＳＰＣ和ＣＰ预处理后， 移植心脏的存活时间明显延长， 炎细胞浸润和心肌细胞变性坏死明显减轻。结论 （１） 急性排斥反应时， 移植心脏内大量的炎细胞浸润和心肌细胞坏死。（２） 大鼠心脏移植的急性排斥反应是由心外膜周围起始逐渐向心内膜方向发展。（３） ＳＰＣ和ＣＰ联合预处理， 可以预防和减缓排斥反应。

Objective The development of myocardial necrosis in cardiac allograft was studied in order to provide theoretical basis for the prevention and treatment of anti rejection.Methods The recipient was pretreated with inoculation of donor splenocytes (SPC) followed by cyclophosphamide( CP). Hematoxylin and eosin (HE) stained section from the allograft was studied about inflammatory cell infiltration and myocardial necrosis.Results Preconditioning with SPC followed by CP could prolong the cardiac allograft survival time. The inflammatory cell infiltration and myocardial necrosis in cardiac allograft were significantly alleviated.Conclusions In acute rejection, there were severe infiltration of many inflammatory cells and myocardial necrosis within cardiac allograft. The development of acute cardiac allograft rejection originated from epicardial area to intimal area of the heart in rat. The pretreatment of SPC followed by CP can prevent and relieve the rejection.