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双重平行检测批量样品对两个实验室RFFIT检测效能的评价 被引量：4

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摘要 快速荧光灶抑制试验(RFFIT)是世界卫生组织(World Health Organisation,WHO)推荐的狂犬病病毒中和抗体检测标准方法之一,国内进行RFFIT检测的不同实验室之间互评可促进该方法推广时的标准化。病毒病预防控制所和中国药品生物制品检定所对200例人血清进行了双重平行RFFIT检测。结果显示双方检测中和抗体效价几何均值(genomic means titration,GMT)为1.89、1.84I U/mL,差别无统计学意义(t=-0.94,P>0.05);血清中和抗体阳性率为95.00%、93.00%,差别无统计学意义(χ2=0.71,P>0.05);双方结果定性一致率为89.00%,发生结果定性不一致的22例(11.00%)血清双方检测结果均<1I U/mL;67例双方检测结果均<1I U/mL的血清样品中有22例(32.84%)发生结果定性不一致。
作者 吕新军 吴小红 李加 于鹏程 申辛欣 吴慧 曹守春 唐建蓉 董关木 唐青
机构地区 病毒病预防控制所 中国药品生物制品检定所
出处 《国际检验医学杂志》 CAS 2011年第4期478-479,共2页 International Journal of Laboratory Medicine
基金 十一五科技重大专项-传染病检测技术研究-传染病病原体诊断和组合检测技术(2008ZX10004-002)
关键词 研究 快速荧光 灶抑制试验 几何均值 一致率 阳性率
分类号 R446.1 [医药卫生—诊断学]
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参考文献10

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二级参考文献28

共引文献54

同被引文献38

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  • 2Smith JS,Yager PA,Baer GM.A rapid reproducible test for determining rabies neutralizing antibody[J].Bull WHO,1973,48(9):535-541.
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  • 6俞永新.中国预防控制狂犬病降低发病率的思考[J].中国计划免疫,2007,13(4):391-398. 被引量：55
  • 7Wang, LY, MP Sun, et al. Safety and Immunogenicity of Two Freeze- dried Vero Cell Rabies Vaccines for Human Use in Post-exposure Prophylaxis [J]. Vaccine, 2011, 29 (15):2679-2681.
  • 82005, WHO Expert Consultation on Rabies, 2004, First Report; WHO Technical Report Series 931. World Health Organization. Geneva, Switzerland.
  • 9Kramer B, Schildger H, et al. The Rapid Fluorescent Focus Inhibition Test is a Suitable Method for Batch Potency Testing of Inactivated Rabies Vaccines[J]. Biologicals, 2009, 37(2): 119-126.
  • 10Smith JS, Yager PA, Baer GM. A Rapid Reproducible Test for Determining Rabies Neutralizing Antibody[J]. Bull WHO, 1973, 48(4): 535-541.

引证文献4

二级引证文献10

国际检验医学杂志
2011年 第4期

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