彗星测定法检测蕈样肉芽肿和Szary综合征的DNA不稳定性

The DNA Instability in Mycosis Fungoides and Sezary′s Syndrome by Using the Comet Assay

目的 研究蕈样肉芽肿 (MF)和S啨ｚａｒｙ综合征 (SS)患者淋巴细胞DNA的不稳定性。 方法采用彗星测定 (CometAssay )或称为单一细胞凝胶电泳 (ThesinglecellgelelectrophoresisSCGE)研究受X线引起MF和SS患者外周血淋巴细胞、皮肤损害的淋巴细胞培养建立的T细胞系的DNA损伤和修复。以正常人淋巴细胞为对照组。结果 MF和SS患者淋巴细胞DNA有明显的不稳定性 ,且很难修复 ,与对照组差异有显著性。结论MF和SS患者淋巴细胞的DNA表现明显的不稳定性和修复障碍 。

Objective To study the DNA instability of the peripheral blood lymphocytes and T cell line from skin lesions of mycosis fungoides(MF) and Sézary′s syndrome (SS).Methods The Comet assay,which was also called as SCGE (the single cell gel electrophoresis), was used to detecting the primary DNA damage at the level of single cell in specimens The DNA damage of X ray irradiated peripheral blood lymphocytes and T lymphocytes from skin lesions, and its repair in lymphoma patients were studied, and compared with those in control persons Results A significantly increased DNA instability in the majority of lymphocytes from the blood and skin lesions of MF and SS patients was observed, and reduced DNA repair of X ray irradiated lymphocytes was found, when compared with the lymphocytes from healthy individuals Conclusion The DNA stability and repairment of both blood and skin homing lymphocytes from patients with MF and SS are significantly impaired, which suggests that MF and SS could develop from a clinically benign skin disease into an overt lymphoma