MMP-9及TIMP-1在子宫颈鳞癌组织中的表达及临床意义

Expressions and clinical significances of MMP-9 and TIMP-1 in cervical squamous cell carcinoma tissues

目的:探讨基质金属蛋白酶9(MMP-9)及基质金属蛋白酶组织抑制因子1(TIMP-1)在宫颈鳞癌组织中的表达,及与宫颈癌临床病理特征的关系。方法:采用免疫组织化学MaxvisioTM法,检测46例宫颈鳞癌,34例宫颈原位癌(CINⅢ)和20例癌旁正常宫颈上皮(NCE)中MMP-9及其抑制因子TIMP-1的表达,分析其表达与宫颈鳞癌的临床分期,病理类型及有无淋巴结转移的关系。结果:MMP-9在宫颈鳞癌,原位癌,NCE中的阳性表达率分别为71.7%,70.6%,25.0%;TIMP-1为54.3%,73.5%,20.0%。MMP-9在NCE组与鳞癌组及原位癌组相比差异有统计学意义(P<0.01),TIMP-1在NCE组与鳞癌组及原位癌组相比差异有统计学意义(P<0.01)。MMP-9和TIMP-1的阳性表达率与临床分期和病理类型均无明显相关性(P>0.05)。MMP-9在淋巴结转移阳性组的阳性表达率明显高于阴性组(P<0.01),而TIMP-1未发现此现象。MMP-9和TIMP-1在宫颈鳞癌中的表达呈负相关(rs=-0.381,P<0.01)。结论:MMP-9在宫颈癌组织中表达水平的上调和TIMP-1的相对弱表达在宫颈鳞癌浸润和转移中起重要的作用。

Objective：To explore the expressions of matrix metalloprpteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-1（TIMP-1） in cervical squamous cell carcinoma tissues,and the relationship between their levels and clinicopathological characteristics of cervical carcinoma.Methods：Immunohistochemical MaxvisioTM method was used to detect the expression levels of MMP-9 and TIMP-1 in 46 cases with cervical squamous cell carcinoma,34 cases with cervical carcinoma in situ（CIN Ⅲ） and 20 cases with normal cervical epithelium adjacent to carcinoma（NCE）.The relationship between their expression levels and clinical stages,pathological types and lymph node metastasis or not were analyzed.Results：The positive expression rates of MMP-9 in cervical squamous cell carcinoma,cervical carcinoma in situ and NCE were 71.7%,70.6% and 25.0%,respectively;the positive expression rates of TIMP-1 in cervical squamous cell carcinoma,cervical carcinoma in situ and NCE were 54.3%,73.5% and 20.0%,respectively.There was significant difference in positive expression rates of MMP-9 and TIMP-1 among the three groups（P〈0.01）.There was no correlation between positive expression rates of MMP-9 and TIMP-1 and clinical stages,pathological types（P〉0.05）.The positive expression rate of MMP-9 in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group（P〈0.01）,but TIMP-1 did not correlate with lymph node metastasis.There was a negative correlation between MMP-9 expression and TIMP-1 expression in cervical squamous cell carcinoma（γs=-0.381,P〈0.01）.Conclusion：The up-regulation of MMP-9 expression and down-regulation of TIMP-1 expression in cervical squamous cell carcinoma tissues play important roles in invasion and metastasis of cervical squamous cell carcinoma.