中国南方人DQB1、DRB1等位基因与妊娠期高血压疾病的关系

The relationship between DQB1 and DRB1 alleles and hypertensive disorder complicating pregnancy in southern areas of China

目的:探讨DQB1及DRB1座位等位基因与妊娠期高血压疾病的关系,阐明妊娠期高血压疾病发生发展的免疫机制。方法:采用快速离心柱法提取对象外周血白细胞DNA,以荧光分光光度计测定DNA含量,应用顺序特异性引物聚合酶链反应技术(PCR-SSP)进行基因测定;取20例妊娠期高血压疾病者作为研究组,18例正常孕妇作为对照组。结果:从单一基因分析,研究组频率显著增高的基因有:DRB1*0303、DRB1*0405、DRB1*0403、DRB1*06011/12、DQB1*0502、DOB1*0402,其中DRB1*0405、DQB1*0502风险处于最高值(RR值分别为9.76、6.34),基因频率显著高于对照组(P<0.05),频率显著降低的基因是:DRB1*0407、DRB1*0315、DQB1*03011/12、DQB1*05031,基因频率显著低于对照组(P<0.05)。2个PIH相关基因组成1个单倍型,其中DRB1*0315-DQB1*0502、DRB1*0405-DOB1*06012单倍型与妊娠期高血压疾病患者尿蛋白及血压呈正相关关系(Pc<0.05)。结论:DRB1和DQB1两座位上等位基因与妊娠期高血压疾病发生密切相关。

Objective：To explore the relationship between DQB1 and DRB1 alleles and hypertensive disorder complicating pregnancy（HDCP）,clarify the immune mechanism of occurrence and development of HDCP.Methods：Rapid centrifugal column method was used to abstract DNA from white blood cells in peripheral blood,fluorescence spectrophotometer was used to detect DNA content,PCR-SSP method was used for gene sequencing;20 cases with HDCP were selected as study group,18 normal pregnant women were selected as control group.Results：According to single gene analysis,the genes with high frequency in study group included DRB10303,DRB10405,DRB10403,DRB106011/12,DQB10502 and DOB10402;the risk values of DRB10405 and DQB10502 were the highest（RR values were 9.76 and 6.34,respectively）,the gene frequencies in study group were significantly higher than those in control group（P〈0.05）;the genes with low frequency in study group included DRB10407,DRB10315,DQB103011/12 and DQB105031,the gene frequencies in study group were significantly lower than those in control group（P〈0.05）.Two PIH related genes constituted one haplotype,there was positive correlation between DRB10315-DQB10502 haplotype,DRB10405-DOB106012 haplotype and HDCP（Pc〈0.05）.Conclusion：DRB1 and DQB1 alleles are related to the occurrence of HDCP.