摘要
目的:探讨DQB1及DRB1座位等位基因与妊娠期高血压疾病的关系,阐明妊娠期高血压疾病发生发展的免疫机制。方法:采用快速离心柱法提取对象外周血白细胞DNA,以荧光分光光度计测定DNA含量,应用顺序特异性引物聚合酶链反应技术(PCR-SSP)进行基因测定;取20例妊娠期高血压疾病者作为研究组,18例正常孕妇作为对照组。结果:从单一基因分析,研究组频率显著增高的基因有:DRB1*0303、DRB1*0405、DRB1*0403、DRB1*06011/12、DQB1*0502、DOB1*0402,其中DRB1*0405、DQB1*0502风险处于最高值(RR值分别为9.76、6.34),基因频率显著高于对照组(P<0.05),频率显著降低的基因是:DRB1*0407、DRB1*0315、DQB1*03011/12、DQB1*05031,基因频率显著低于对照组(P<0.05)。2个PIH相关基因组成1个单倍型,其中DRB1*0315-DQB1*0502、DRB1*0405-DOB1*06012单倍型与妊娠期高血压疾病患者尿蛋白及血压呈正相关关系(Pc<0.05)。结论:DRB1和DQB1两座位上等位基因与妊娠期高血压疾病发生密切相关。
Objective:To explore the relationship between DQB1 and DRB1 alleles and hypertensive disorder complicating pregnancy(HDCP),clarify the immune mechanism of occurrence and development of HDCP.Methods:Rapid centrifugal column method was used to abstract DNA from white blood cells in peripheral blood,fluorescence spectrophotometer was used to detect DNA content,PCR-SSP method was used for gene sequencing;20 cases with HDCP were selected as study group,18 normal pregnant women were selected as control group.Results:According to single gene analysis,the genes with high frequency in study group included DRB10303,DRB10405,DRB10403,DRB106011/12,DQB10502 and DOB10402;the risk values of DRB10405 and DQB10502 were the highest(RR values were 9.76 and 6.34,respectively),the gene frequencies in study group were significantly higher than those in control group(P〈0.05);the genes with low frequency in study group included DRB10407,DRB10315,DQB103011/12 and DQB105031,the gene frequencies in study group were significantly lower than those in control group(P〈0.05).Two PIH related genes constituted one haplotype,there was positive correlation between DRB10315-DQB10502 haplotype,DRB10405-DOB106012 haplotype and HDCP(Pc〈0.05).Conclusion:DRB1 and DQB1 alleles are related to the occurrence of HDCP.
出处
《中国妇幼保健》
CAS
北大核心
2011年第10期1532-1535,共4页
Maternal and Child Health Care of China
关键词
HLA等位基因
妊娠期高血压疾病
基因频率
HLA allele; Hypertensive disorder complicating pregnancy; Gene frequency;