摘要
目的:探讨人子宫颈病变组织中P53的表达及其与端粒酶hTR mRNA表达的相关关系。方法:51例宫颈病变组织中,包括宫颈炎10例,LSIL10例,HSIL20例,SCC11例,分别利用免疫组化S-P法和原位杂交法检测病变组织中P53蛋白、端粒酶hTRmRNA的表达活性。结果:P53表达在宫颈炎组与LSIL组无差别,但显著低于HSIL和SCC组;hTR基因在HSIL/SCC中的阳性表达与CC/LSIL组以及在LSIL和HSIL/SCC之间差异有统计学意义(P<0.05);P53蛋白表达与端粒酶hTRmRNA基因表达之间具有显著相关性(P=0.042,P<0.05)。结论:端粒酶hTR和P53蛋白表达及相关性对于人子宫颈癌的早期病理诊断有较好地应用价值。同时,对该肿瘤的发生、发展规律提供一定的客观依据。
Objective：To explore the expression of P53 protein in human cervical lesion tissues and its relationship with telomerase hTR mRNA.Methods：51 samples of cervical lesion tissues were selected,including 10 samples of cervicitis,10 samples of low grade squamous intraepithelial lesion（LSIL）,20 samples of high grade squamous intraepithelial lesion（HSIL） and 11 samples of squamous cell carcinoma（SCC）;immunohistochemical SP method and in situ hybridization were used to detect the expression levels of P53 protein and telomerase hTR mRNA.Results：There was no significant difference in expression level of P53 protein between cervicitis group and LSIL group,but the expression levels of P53 protein in cervicitis group and LSIL group were significantly lower than those in HSIL group and SCC group;there was significant difference in expression level of telomerase hTR mRNA between HSIL/SCC group and cervicitis/LSIL group,as well as between LSIL group and HSIL/SCC group（P〈0.05）.There was a significant correlation between P53 protein expression and telomerase hTR mRNA expression（P=0.042,P〈0.05）.Conclusion：Telomerase hTR mRNA expression and P53 protein expression and their correlation are of great application value in early pathological diagnosis,which provide a certain objective basis for the oncogenesis and development of cervical carcinoma.
出处
《中国妇幼保健》
CAS
北大核心
2011年第10期1539-1542,共4页
Maternal and Child Health Care of China
基金
深圳市科技计划项目〔200204068
200802033〕
