高效液相色谱法测定烟草中水溶性糖

HPLC Determination of Water Soluble Saccharides in Tobacco

提出了高效液相色谱法测定烟草中水溶性糖含量的方法。烟草样品经0.01mol·L^(-1)氢氧化钠溶液振荡提取60 min,以Metacarb 67C色谱柱(300mm×6.5mm)为固定相,纯水为流动相等梯度洗脱,用示差折光检测器检测。烟草中主要的水溶性糖均在12 min内达到基线分离,果糖、葡糖糖和蔗糖的检出限(3S/N)分别为0.60,0.71,0.39 mg·L^(-1)。方法加标回收率在98.1%～99.2%之间,相对标准偏差(n=8)在0.97%～1.6%之间。

A method of HPLC for the determination of water soluble saceharides in tobacco was proposed. The sample was extracted vortically with 0. 01 mol·L^-1 NaOH solution for 60 min, and the extract obtained was separated by isogradient elution with pure water on Metacarb 67C column （300 mm× 6. 5 mm）, and determined with differential refraction detector. The main water soluble saccharides in tobacco were successfully separated within 12 min, and detection limits （3S/N） found were 0. 60 mg ·L^-1 for fructose, 0. 71 mg ·L^-1 for glucose and 0. 39 mg·L^-1 for sucrose Values of recovery and RSD＇s （n=8） of the 3 water soluble saccharides found were in ranges of 98. 1%-99.2% and 0. 97%-1.6% respectively.