盐酸鸟嘌呤与牛血清白蛋白的相互作用

Interaction Between Guanine Hydrochloride and Bovine Serum Albumin

应用荧光光谱、紫外-可见分光光度法研究了盐酸鸟嘌呤(GH)与牛血清白蛋白(BSA)的相互作用。结果表明:GH能猝灭BSA的荧光强度,其猝灭机理为静态猝灭。采用位点结合模型公式和热力学公式计算了结合常数、结合位点数及结合类型。用同步荧光技术研究GH对BSA构象的影响。

Mechanism of the interaction between guanine hydrochloride （GH） and bovine serum albumin （BSA） was studied by fluorospectrometry and UV-VIS spectrophotometry. Quenching of fluorescence of BSA was observed due to its reaction with GH, and the interaction was found to be a static quenching process. The binding constants, number of binding sites and types of binding force were found by applying the equation of site-binding model and thermodynamic equations. Synchronous fluorescence spectrometry was used in the study of the effect of GH on the configuration of BSA.