不同方法接种构建的可移植性小鼠L1210白血病模型的生物学特性

Biological features of the transferable mouse L1210 leukemia model constructed by different inoculation methods

目的对不同接种方法构建的小鼠L1210白血病模型的某些生物学特性进行比较研究，选择基本符合L1210细胞生物学特性，且一致性更好的接种方法。方法常规培养小鼠急性淋巴细胞白血病细胞系L1210，分别给DBA／2小鼠以静脉（A组）、皮下（B组）及腹腔注射（c组）3种途径接种，观察小鼠成瘤情况，定期计数各组小鼠外周白细胞数量并观察细胞形态改变，取濒死小鼠的肝、脾、肺、肾等脏器称重并制作病理切片。结果各组小鼠成瘤率均为100％，A、B、C3组成瘤天数分别为：14、7和14d；平均存活天数分别为：（25．3±3．10）、（25．6±1．92）、（18．1±1．87）d。接种后2周外周恤白细胞数目分别增加至（18．32±2．01）、（22．20±3．88）、（27．96±7．13）×10^9／L，接种后3周外周血白细胞数目分别增加（22．01±11．96）×10^9／L（A组）和（70．23±3．04）×10^9／L（B组）；外周血涂片中均可见白血病细胞；各组濒死小鼠肝、脾有弥漫性白血病细胞浸润，正常组织结构破坏；肺和肾内有少量白血病细胞浸润，正常组织结构破坏不明显，肺脏有明显出血。结论静脉、皮下、腹腔注射体外培养的L1210细胞于DBA／2小鼠均可成瘤。皮下注射成瘤时间短，存活时间长，可见明显的瘤结节；腹腔注射成瘤时间长，存活时间短，与L1210细胞传代的接种方式一致；静脉注射成瘤时间长，存活时间也长，与白血病血行播散的方式一致。

Objective To make a comparative study of biological characteristics of a transferable mouse L1210 leukemia model inoculated differently in order to select the best inoculation method that fits in with the bionomics of L1210 cells and has a better consistency. Methods The mouse acute lymphoblastic leukemia cell line L1210 was conventionally infused into each DBA/2 mouse through the vena caudalis （ A group）, subcutis （ B group） and abdominal cavity （ C group）. The development of leukemia in these mice was investigated. The amount of peripberial leucocytes in mouse blood was counted and the morphological changes were investigated. The liver, spleen, kidney and lung of the dying mice were kept, weighed and made into pathological sections. Results The rate of tumorrigenesis, which took 14,7 and 14 days respectively, was 100% in each group. The average survival time of the three groups was （25.3 ±3.10） , （25.6 ± 1.92） , and （ 18.1 ± 1.87） days. The amount of peripherial leucocytes increased remarkably after infusion, which was （18.32 ±2.01） ,（22.20 ±3.88） and （27.96 ±7.13） × 10^9/L after two weeks and （22.01 ± 11.96） × 10^9/L （A group） and （70.23 ± 3.04） × 10^9/L （B group） after three weeks. Leukemic cells could be found in peripheral blood smears of the three groups. In pathological sections of the liver and spleen of dying mice, diffused leukemic cell infiltration could be observed, accompanied by destruction to the normal tissue and structure. A small quantity of leukemic cells was found in lungs and kidneys. The destruction to their normal tissue and structure was not obvious and hemorrhage was easily seen in lungs. Conclusions Inoculation of L1210 cells into DBA/2 mice through the vena eaudalis, subcutis and abdominal cavity is feasible for developing a mouse leukemia model. By hypodermic injection, the duration of tumor formation is short, the survival time is long and tumor nodules are visible. By intraperitoneal injection, the duration of tumor formation is long and survival time is short, conforming to the way L1210 cells are passed. By intravenous injection, both the duration of tumor formation and survival time is long, consistent with the way leukemia cells are hematogenously disseminated.