反复自然流产患者封闭抗体检测与免疫治疗分析

Antipaternal lymphocyte antibody in patients with spontaneous abortion and immunotherapy analysis of 133 cases

目的评价反复自然流产(recurrent spontaneous abortion,RSA)患者封闭抗体(blocing antibodies,BA)水平,观察应用淋巴细胞免疫疗法的治疗效果。方法采用酶联免疫吸附试验(ELISA)检测133例RSA患者血清的BA;对我院33例BA阴性、反复自然流产患者的丈夫进行淋巴细胞免疫治疗。结果反复自然流产患者BA阴性率为88.7%,免疫治疗的33例患者,复查BA 32例转阳性,阳性率为96.96%。12例再次妊娠,1例再次发生流产。结论反复自然流产与患者BA缺乏有关,淋巴细胞免疫治疗反复流产安全、有效。

Objective To detect the level of the blocking antibody（BA） and evaluate the efficiency of immunotherapy for in fertile patients with recurrent spontaneous abortion. Methods The blocking antibody was detected by enzyme - linked immunosorbent assay （ELISA） in 133 patients with primary recurrent spontaneous abortion, Thirty -three RSA women whose blocking antibodies were negative were immunized with lymphoeytes. Results The negative rate of blocking antibodies in patients with recurrent spontaneous abortion was 88.7%. After immunotberapy, all of the thirtythree patients with negative blocking antibodies turned positive but one. Conclusion Recurrent spontaneous abortion is correlated with the lack of blocking antibodies. Immunotherapy with lymphoeytes is effective in treating RSA.