乳腺癌激素水平和P53癌基因的表达及意义

HORMONE LEVELS AND EXPRESSION OF P53 GENE OF BREAST CANCER AND ITS CLINICAL SIGNIFICANCE

目的 研究乳腺癌患者雌孕激素水平和Ｐ５３ 基因蛋白的表达及其临床意义。方法 应用放射免疫法对６８ 例乳腺癌和５０ 例乳腺良性病变分别测定其血清雌孕激素水平，免疫组化ＡＢＣ 法检测Ｐ５３ 基因蛋白在其病理切片上的表达。结果 雌激素水平乳腺癌组明显高于乳腺良性病变组（ Ｐ＜００１） ，而孕激素水平差异不大；Ｐ５３ 基因蛋白的阳性表达率乳腺癌组显著高于良性病变组（ Ｐ＜ ０００５） 。乳腺癌组雌激素水平腋淋巴结阳性者显著高于阴性者（ Ｐ＜ ０００５） ，Ｐ５３ 基因蛋白的阳性表达与乳腺癌组织学分级（ Ｐ＜ ０００５） 、临床分期显著正相关（ Ｐ＜ ００５） ；乳腺癌术后５ 年内复发或死亡者的雌激素水平明显高于无瘤生存者（ Ｐ＜ ００５） ，Ｐ５３ 基因蛋白阳性表达者术后５ 年无瘤生存率显著低于Ｐ５３ 表达阴性者（ Ｐ＜ ００１） 。结论 雌激素水平过高和Ｐ５３ 基因的阳性表达是乳腺癌患者表现不佳和预后不良的指标，雌激素水平和Ｐ５３

Objective: To reveal clinical significance and the relationship between clinical character of breast cancer and hormone levels, and between clinical character and espression of P53 gene. Methods: Using radioimmunologic techniques, we detected the serum hormone levels, and using immunohistochemistric techniques, detected the expression of P53 gene in 68 cases of breast cancer and 50 cases of breast benign. disease. Results: The estradial level of breast cancer was higher than that of breast benign disease( P<0 01 ),but the progesterone had no difference. The rate of P53 gene expression of breast cancer was higher than benign ( P<0 005 ). In breast cancer the estradial level of positive lymph node was higher than negitive ( P<0 005 ). Significant relationships were seen between P53 Positive expression and histologic grades ( P<0 005 ),and between P53 and clinical INM staging ( P<0 005 ). In postoperative five years, the estradial level of recurrent or died cases was higher than cancer-free existence ones ( P<0 005 ). However the rate of five years cancer-free existence case of positive expression of P53 gene was significant lower than negative P53 expression ( P<0 01 ). Conclusion The high estradial level and positive expression of P53 gene were indicators for character and poor prognosis of breast cancer, and there were direct significance for its treatment and prognostic judgment.