摘要
目的：在多因素串联色谱响应函数（ＨＣＲＦ） 基础上提出了新的优化指标ｇＨＣＲＦ，对新化合物氯苄律定有关物质的ＨＰＬＣ 分离进行优化。方法：采用球面对称设计，对影响色谱分离的两个主要因素（ｘ１ ：甲醇比例；ｘ２ ：离子对试剂浓度） 进行优化，并对试验结果进行关系式模拟。结果：以ｇＨＣＲＦ 建立的关系式预测的理论最优解为ｘ１ ＝７６ ％ ，ｘ２ ＝ ０－０１ ｍｏｌ·Ｌ－ １ ，与实验最优解ｘ１ ＝７５％ ，ｘ２ ＝ ０－０１ ｍｏｌ·Ｌ－１ 十分接近，预测能力可信。
AIM: The generalized HCRF (gHCRF) was proposed as a new optimization parameter based on heterogeneous chromatographic response function(HCRF), and was first used in the HPLC separation of chrolobenzylidine and its related compounds. METHODS: By use of Spherical Symmetric Design, optimization was made on the two factors ( x 1: methanol concentration; x 2: perchloric acid concentration) which affected the chromatographic separation. The equations simulating the relationship between response values and factors were setup. RESULTS: The theoretical optimal condition from gHCRF equation is x 1=76% and x 2=0 01 mol·L -1 , which is very much close to the experimental optimal condition ( x 1=75% and x 2=0 01 mol·L -1 ). Therefore, the prediction from the equation is reasonable. CONCLUSION: The separation of chrolobenzylidine and its related compounds showed that the gHCRF parameter can be satisfactorily applied to the chromatographic optimization of complex samples.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第10期772-775,共4页
Acta Pharmaceutica Sinica
基金
国家新药研究基金
国家医药科技创新1035 项目
关键词
氯苄律定
色谱优化
gHCRF
球面对称设计
chrolobenzylidine
chromatographic optimization
generalized heterogeneous chromatographic response function(gHCRF)
spherical symmetric design
