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分光光度法快速测定硝、铵态氮的可行性研究 被引量:7

Feasibility Study on NO_3^--N,NH_4^+-N Determination by Spectrophotometry Method
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摘要 用紫外分光光度法、靛酚蓝分光光度法测定北方4类土壤硝、铵态N的含量,并对测试条件进行可行性研究。结果表明:紫外分光光度法测定土壤硝态N时需反应30 min后进行测定,温度对紫外分光光度法硝态N测定值没有明显影响;靛酚蓝分光光度法分析土壤样品中的铵态N时,至少要放置10 h,并且要保持测定样品和制定工作曲线时的温度相差不大。 NO3--N,NH4+-N contents of four kinds of soil samples from north were determined by uv-spectorophotometry and indophenol blue colorimetric and the feasibility of measure conditions were studied.The results showed that determination of soil NO3--N started after the reaction lasted 30 min.Effect of temperature on the measured values of NO3--N by uv-spectrophotometry was no significant.The samples needed to be placed at least 10 hours when NH4+-N content was determined by indophenol blue colorimetric,meanwhile,the temperature of samples and curve formulation must only have minor differences.
作者 赵洁 王莉
出处 《现代农业科技》 2011年第6期42-42,45,共2页 Modern Agricultural Science and Technology
基金 国家科技支撑计划重点项目(2008BADA4B01)
关键词 硝态N 铵态N 紫外分光光度法 靛酚蓝分光光度法 NO3--N NH4+-N uv-spectrophotometry indophenol blue colorimetric
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