HPLC法测定蒲葵药材中6种组分含量

HPLC determination of the six components in Livistona chinensis

目的:建立HPLC法同时测定蒲葵中荭草苷、异荭草苷、牡荆苷、异牡荆苷、异鼠李素-3-O-β-D-葡萄糖苷、苜蓿素含量,并比较不同产地不同部位蒲葵中6种组分的含量。方法:采用Agilent TC-C18(4.6 mm×250 mm,5μm)色谱柱;以甲醇(B)-0.05%醋酸溶液(A)为流动相,梯度洗脱(0～25 min,B:33%→45%;25～40 min,B:45%→80%;40～45 min,B:80%→95%;45～50 min,B:95%→95%;50～60 min,B:95%→33%);流速0.800 mL·min-1;检测波长340 nm,柱温30℃。结果:6个成分的检测范围分别为荭草苷:8.59～550.0μg·mL-1(r=0.9997)、异荭草苷:9.37～600.0μg·mL-1(r=0.9999)、牡荆苷:8.59～550.0μg·mL-1(r=0.9998)、异牡荆苷:8.59～550.0μg·mL-1(r=0.9995)、异鼠李素-3-O-β-D-葡萄糖苷:7.81～500.0μg·mL-1(r=0.9999),苜蓿素:8.59～550.0μg·mL-1(r=0.9999);平均回收率(n=3)分别为102.9%(RSD=4.6%),102.3%(RSD=2.3%),93.3%(RSD=2.1%),95.8%(RSD=2.7%),101.7%(RSD=1.8%),99.2%(RSD=2.5%)。结论:本方法具有良好的重复性,为评价蒲葵药材的质量提供了分析方法。不同产地不同部位的含量存在显著性差异。

Objective：To develop an HPLC method to determinate the proportion of orientin,isoorientin,vitexin,isovitexin,isorhamnetin-3-O-β-D-glucoside,tricin of different parts in Livistona chinensis from different origins.Method：The separation was performed on an Agilent TC-C18（4.6 mm×250 mm,5 μm） with a gradient elution（0-25 min,B：33%→45%;25-40 min,B：45%→80%;40-45 min,B：80%→95%;45-50 min,B：95%→95%） composed of methanol（B） and 0.05% acetic acid（A）.The column temperature was set at 30 ℃ with flow rate of 0.8 mL·min-1.The detective wavelength was set at 340 nm.Results：The calibration curve was linear over the concentration of 8.59-550.0 μg·mL-1 for orientin（r=0.9997）;9.37-600.0 μg·mL-1 for isoorientin（r=0.9999）;8.59-550.0 μg·mL-1for vitexin（r=0.9998）;8.59-550.0 μg·mL-1 for isovitexin（r=0.9995）;7.81-500.0 μg·mL-1 for isorhamnetin-3-O-β-D-glucoside（r=0.9999）;8.59-550.0 μg·mL-1 for tricin（r=0.9999）.The average recovery of six components were 102.9%,102.3%,93.3%,95.8%,101.7%,99.2% and the RSDs were 4.6%,2.3%,2.1%,2.7%,1.8% and 2.5%,respectively.Conclusion：The method is reproducible,and can be used for the quality control of Livistona chinensis.The yields of the six components in different parts of different origins exhibite significant differences.