HPLC法同时测定糙苏中3个苯乙醇苷类化合物含量

HPLC simultaneous determination of three phenylethanoids in Phlomis umbrosa

目的:建立同时测定糙苏中3个苯乙醇苷含量的HPLC法。方法:采用Agilent Zorbax SB C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-磷酸二氢钾缓冲盐(pH=2.5)20∶80等度洗脱,流速1.0 mL·min-1,检测波长334 nm。结果:连翘酯苷B在0.32～1.60μg,类叶升麻苷在0.48～2.40μg,异类叶升麻苷在0.36～1.80μg与峰面积呈良好的线性关系;r分别为0.9999,0.9995,0.9998。平均加样回收率(n=3)分别为99.6%,99.4%,98.6%;RSD分别为0.88%,0.40%,1.0%。结论:本方法快速简便、灵敏、可靠,为土家族民间药物糙苏的质量评价提供了科学依据。

Objective： To establish an HPLC method for simultaneous determination of forsythoside B,verbascoside and isoverbascoside in Phlomis umbrosa.Methods：The separation was performed on an Agilent Zorbax SB C18 column（4.6 mm×250 mm,5 μm）,using acetonitrile and water（potassium dihydrogen phosphate solution,pH 2.5） 20∶80 as the mobile phase.The flow rate was 1.0 mL·min-1;The detection wavelength was 334 nm.Results：The linear ranges of forsythoside B,verbascoside and isoverbascoside was 0.32-1.60 μg（r=0.9999）and 0.48-2.40 μg（r=0.9995）and 0.36-1.80 μg（r=0.9998）,respectively,the average recoveries（n=3） was 99.6% with RSD of 0.88%,99.4% with RSD of 0.40%,98.6%with RSD of 1.0%,respectively.Conclusion：The method is simple,accurate and can be used for quality control of Phlomis umbrosa.