不同方法炮制对巫山淫羊藿中主要成分朝藿定C和淫羊藿苷的影响

Effects of different processed products on the main ingredient of epimedin C and icariin of Epimedium wushanense

目的:研究不同方法炮制对巫山淫羊藿中主要成分朝藿定C及淫羊藿苷的影响。方法:采用RP-HPLC法,使用Shim-pack VP-ODS(5μm,150 mm×4.6 mm)色谱柱,以乙腈-水(25∶75)流动相,流速为1 mL·min-1,检测波长270 nm,柱温30℃,比较不同炮制品中朝藿定C及淫羊藿苷含量的变化。结果:巫山淫羊藿不同炮制品中活性成分淫羊藿苷含量均有较大程度的上升;而朝藿定C变化则比较复杂,除羊脂油炙巫山淫羊藿中朝藿定C含量有较大的下降外,其余均有较小程度的上升。结论:加热炮制可能促使巫山淫羊藿中的其他成分分解或转化为淫羊藿苷;羊脂油炙巫山淫羊藿中朝藿定C含量有较大的下降,提示朝藿定C可能不是淫羊藿中的助阳成分;不同方法炮制对巫山淫羊藿主要成分影响较大,提示巫山淫羊藿在临床应用时必须选择合适的炮制方法。

Objective：To study effects on the main ingredient of epimedin C and icariin of Epimedium wushanense T.S.Ying of different processed products.Methods：Epimedin C and icariin were separated on Shim-pack VP-ODS（5 μm,150 mm×4.6 mm） column,the mobile phase was 25% acetonitrile aqueous solution at a flow rate of 1 mL·min-1,the detection wavelength was set at 270 nm and column temperature was 30 ℃.The content changes of epimedin C and icariin of E.wushanense processed by different methods was compared.Results：Active ingredients of icariin had a greater degree of increase of different processed products of E.wushanense,while epimedin C change was more complex and,in addition to sheep-fat-broiled product of E.wushanense content of epimedin C would have a greater down,the rest was a lesser extent.Conclusion：Different processed products may prompt other components break down or converted into icariin of E.wushanense;there is a greater content decline of epimedin C of sheep-fat-broiled product of E.wushanense,suggesting epimedin C may not be help Kidney-Yang elements;there is significant difference for the major components of E.wushanense by different processing methods,selection the appropriate processing methods is necessary in clinical application.