缺氧诱导因子-1α(HIF-1α)与P糖蛋白(P-gp)在胆管癌中表达及相关性研究

Experssion of hypoxia-inducible factor 1(HIF-1α)and P-glycoprotein(P-gp)in cholangiocarcinoma and their correlation

目的:研究缺氧诱导因子-1α(HIF-1α)与P糖蛋白(P-gp)在胆管癌中的表达并探讨其临床意义及两者之间的相关性。方法:采用SP免疫组化法检测胆管癌组织及正常胆管组织中HIF-1α与P-gp的表达。应用SSPS17.0统计软件进行数据处理。结果:HIF-1α和P-gp在胆管癌中的阳性表达率为68.9%和62.2%,均显著高于两者在正常胆管组织中的表达,差异有统计学意义(P<0.05)。胆管癌中HIF-1α的表达与淋巴结转移有关(P<0.05),P-gp的表达与肿瘤的分化程度有关(P<0.05),它们的表达与性别、年龄、部位及TNM分期无关(P>0.05)。HIF-1α与P-gp的表达呈正相关关系(P<0.05)。结论:HIF-1α与P-gp在胆管癌组织中高表达,且它们的表达呈正相关性。对两者的共同检测可评估临床化疗疗效及患者预后。

Objective To investigate the expression of HIF-1αand P-gp in cholangiocareinoina and to explore the correlation between HIF- 1αand P-gp .Methods hnmunohistochemical SP method was used to examine the expression of HIF-1α and P-gp in cholangiocarcinoma and norreal extrahepatic bile duct.Resuhs The positive expession rates of HIF-1 αand P-gq3 were 68.9%and 62.2% respectively in cholangiocarcinoma tissues,both of which were significantly higher than those in normal extrahepatic bile duct （P〈0.05）.The expression of HIF-1α was related with lymph node metastasis（P〈0.05）and the expression of P-gp was related with the differrntiation degree（P〈0.05）.The expressions of HIF-1α and P- gp was not related with sex,age,siz and TNM Typing（P〈0.05）.The expression of HIF-1α had positive correlation with the expression of P-gp （P〈 0.05）.Conclusion The high expressions of HIF-1α and P-gp are observed in cholangiocarcinoma and they are positive correlated.Combined detection of the two indicators can evaluation of the clinical efiects of chemotherapy and the prognosis in patients with cholangiocarcinoma.