分子印迹膜SPR传感器检测氯磺隆的方法

Recognition of Chlorsulfuron Based on SPR Sensors with the Molecular Imprinted Polymer

研究使用了中国科学院电子学研究所自行研制的高灵敏度单通道SPR分析仪和进样装置,仪器为棱镜耦合型SPR传感器结构,其检测角度范围是40°～70°,折射率检测范围为1.04～1.47,谐振角的精度在0.001°。适用于SPR分析仪的分子印迹芯片采用PVC-MIP共聚膜法制备,SPR角度扫描结果表明,200nm以下厚度芯片具有较好的SPR吸收特性。以氯磺隆为模板的分子印迹膜与不加氯磺隆的分子印迹膜对比实验发现前者具有特异性结合的能力。实验对浓度0.1,0.2,0.5和1μg/mL的氯磺隆进行了SPR定点检测,这四种浓度氯磺隆的折射率响应信号满足线性关系,相关系数R=0.996 4。实验中对低浓度的氯磺隆进行反复检测,检测到50ng/mL的氯磺隆,满足农残检测的要求。

The high sensitivity single channel surface plasmon resonance（SPR） instrument and sample injection device made by IECAS were used in this research.The SPR instrument is of prism coupled structure with the incident angle of 40°-70°,the refractive index of 1.04-1.47,and the precision of the resonant angle of 0.001°.The molecular imprinted polymer（MIP）-SPR chip was made by PVC-MIP method and the chip thinkness below 200 nm was proved to have an excellent resonant angle.The MIP chip with the chlorsulfuron（CS） template has the ability for special adsorption compared with the non-MIP chip.The SPR detections for the CS concentrations of 0.1,0.2,0.5 and 1 μg/mL were carried out,and the refractive response signal of them meets a linear equation with R=0.996 4.The detection for the low-concentration CS was made repeatedly.The detection results show that the 50 ng/mL CS satisfies the requirement of the pesticide residue detection.