九香止泻片对湿热泄泻型肠易激综合征大鼠肠道组织肥大细胞与五羟色胺表达的影响

Effect of Nine Enteric Tablets on Mast Cells and 5-HT Expression of Irritable Bowel Syndrome Rats with Damp-Heat Diarrhea Type

目的通过肥大细胞(MC)与五羟色胺(5-HT)在肠易激综合征(IBS)大鼠肠道组织的表达改变,探讨九香止泻片的作用机制。方法选用40只SD大鼠,随机分为空白组、模型组、实验组、对照组,每组10只。除空白组外,其余各组均予以胃湿热方法造模,同时再予以IBS慢性应激造模。造模至第22日开始,实验组予以九香止泻片灌胃,原药材量12.27 g/(kg.d);对照组予盐酸洛哌丁胺灌胃1.08 mg/(kg.d),每日1次,连续灌胃7 d。实验结束时观察各组大鼠肠道中MC与5-HT的表达等改变。结果实验组在抑制湿热泄泻型IBS大鼠肠道中MC与5-HT表达等方面与模型组比较,差异有统计学意义(P<0.05)。结论九香止泻片能够抑制湿热泄泻型IBS模型大鼠肠道(回盲部、结肠远端)中的5-HT及MC的异常表达,从而达到止泻的目的。

Objective To explore the mechanism of Nine Enteric tablets by observing the mast cells and 5-HT expression of irritable bowel syndrome fIBS） rats. Methods Forty SD rats were divided into four groups： blank group, model group, experimental group, control group, 10 in each group. Except for the blank group, other groups were made for stomach damp-heat, and then for IBS with chronic stress. The experimental group was administrated with Nine Enteric tablets 12.27 g/（kg.d） and the control group with loperamide hydrochloride 1.08 mg/（kg . d） once per day for 7 d. The intestinal mast cells and 5-HT expression were observed. Result Experimental group inhibited intestinal mast cells and 5-HT expression of damp-heat diarrhea type IBS rats compared with model group, the difference was significant （P 〈0.05）. Conclusion Nine Enteric tablets can inhibit abnormal expression of intestinal （ileocecal, colon distal） mast cells and 5-HT of damp-heat diarrhea type IBS rat model, and thus achieve the purpose of relieving diarrhea.