摘要
目的采用高效液相色谱法测定当归养血丸中阿魏酸的含量。方法采用高效液相色谱法,Diamonsil ODS1 C18色谱柱,以乙腈-0.085%磷酸溶液(17∶83)为流动相,流速为1.0 mL/min,检测波长316 nm。结果阿魏酸在0.649 4~6.494μg范围内呈良好线性关系,回归方程为Y=354 596.72X+5 347.81,r=0.999 9。平均加样回收率98.8%,RSD=0.89%(n=6)。结论本方法简便、准确,专属性强,测定结果重复性好,为当归养血丸中阿魏酸的定量分析提供了有效方法。
Objective To establish the method for detemining the content of Ferulic acid in Danggui Yangxue Wan by HPLC. Methods HPLC method was adopted on Diamonsil ODS1 C18 column, with acetonitrile-0.085% phosphoric solution (17 : 83) as the mobile phase, detection wavelength as 316 rim, and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.6494-6.494μg for Ferulic acid and linear equation was Y=354 596.72X+5 347.81, r =0.999 9. The average recovery was 98.8% and the related standard deviation (RSD) was 0.89% (n =6). Conclusion This method was simple, accurate and proper, and the reduplication of the result was good. It provides effective method for quantitative analysis of Ferulic acid in Danggui Yangxue Wan.
出处
《中国中医药信息杂志》
CAS
CSCD
2011年第4期47-48,共2页
Chinese Journal of Information on Traditional Chinese Medicine