利用SSR标记划分黑龙江省中早熟玉米自交系杂种优势群的研究

Studies on Heterotic Group of Middle Early Mature Maize Inbred Lines in Heilongjiang by SSR Markers

利用SSR标记研究了25个玉米自交系的遗传变异,初步进行了杂种优势群划分。从100对SSR引物中筛选出80对扩增产物具有稳定多态性的引物,80对引物在供试材料中共检测出361个等位基因变异,平均每对引物检测到等位基因数位4.513个,变化范围在2～8个,多态性信息含量变化范围为0.298～0.827,平均多态性信息量为0.615。25个自交系之间的遗传相似系数变化范围为0.554～0.939。UPGMA聚类分析结果表明,供试自交系可分为4大类群(6个亚群),分类结果与系谱基本一致。

The genetic diversity of 25 middle early mature maize inbred lines in Heilongjiang was analyzed by SSRs,and the heterotic groups were classified.Eighty pairs of primers whose bands were clearly amplified and discrepant were selected out of 100.Totally 361 alleles were identified,and the average number of alleles per SSR locus was 4.513 in these lines with a range from 2 to 8.Polymorphism information content（PIC） values varied from 0.298 to 0.827 with an average of 0.615.The genetic similarity coefficient（GS） ranged from 0.554 to 0.939 among 25 inbred lines.By clustering analysis,25 inbred lines were divided into 4 heterotic groups.Cluster analyses by UPGMA were basically corresponding to their pedigree analysis.