复方中药促进创伤愈合作用效果研究

The therapeutic effect of compound traditional Chinese medicine prescription on wound healing in mice

通过复方中药对创伤愈合的作用效果研究,初步筛选出一种促进伤口愈合的中药方剂。试验选取蒲公英、金银花、丹参等12种中草药,依据清热解毒、活血止血等组方原则组成甲、乙、丙3个复方,并制成膏剂。以小白鼠背部皮肤创伤为动物模型,以伤口愈合面积、愈合率、愈合时间、病理变化作为疗效指标,与莫匹罗星软膏(对照组)对照,观察各复方对创伤愈合的作用效果。结果表明,实验组与空白组(不做任何处理)比较,各复方中药能明显提高小白鼠的伤口愈合率(P<0.05),复方中药乙组与对照组比较,伤口愈合率无明显差异(P>0.05),而显著高于复方中药甲、丙组(P<0.05);与空白组比较,实验组和对照组均能促进表皮生成,减少炎性细胞浸润,加速胶原纤维增生和促进瘢痕形成,但复方乙组愈合效果最佳。提示复方乙组与西药疗效近似,其次是丙组,甲组效果最差;筛选出的复方中药乙能减少炎性细胞浸润,加速胶原纤维增生,防止伤口感染,在创伤愈合中疗效显著。

To study the therapeutic effect of compound traditional Chinese medicine prescription（vulneraria） on the wound,12 kinds of Chinese herbs,such as dandelion,honeysuckle and salvia,etc.,were composed of three prescriptions based on clearing away heat and toxic material,promoting blood circulation,arresting bleeding and other principles,and made them into paste.Mice models with skin injury were established and the area of wound healing,curative rate,curative time and pathological sections were used as indicator to estimate the therapeutic effect of compound traditional Chinese medicine with Mupirocin ointment as control.Results： The wound healing rate of t the vulneraria group was markedly higher than that of the vacant group（P〈0.05）.There was no significant difference in the wound healing rate between the vulneraria group and the sulfadiazine silver group（P〈0.05）.Both of the vulneraria and the Mupirocin ointment could promote the formation of epidermis,decrease the inflammatory cell infiltration and accelerate the proliferation of fibroblasts,resulting in scar formation.The effect of compound Chinese medicine B was the best.Conclusions： Effect of compound Chinese medicine B was similar to western medicine,and followed by C and A.Chinese vulneraria B may play a significant role in skin wound healing by decreasing the inflammatory cell infiltration,accelerating the proliferation of fibroblasts and protecting the wound from infection.