生长抑素受体特异性激动剂对人肝癌细胞生长影响的实验研究

The Effects of Somatostatin Receptor Subtype Specific Agonists on Human Hepatocellular Carcinoma Cell

目的探讨生长抑素受体特异性激动剂对体外培养的人肝癌细胞HepG2生长的影响。方法分别采用MTS、TUNEL、Transwell法检测生长抑素受体亚型特异性激动剂对体外培养的人肝癌细胞株HepG2增殖、凋亡、迁移的影响。结果与对照组相比,SSTR2~4亚型特异性激动剂对人肝癌细胞的增殖、凋亡、迁移没有明显影响,SSTR5亚型特异性激动剂L-817、818在剂量为1μg·mL-1时可以出现较明显的迁移抑制作用,剂量达到5μg·mL-1时,差异有统计学意义（P〈0.05）。其他不同浓度激动剂对HepG2的迁移抑制作用不明显。结论 SSTR亚型特异性激动剂对肝癌细胞的增殖、凋亡、侵袭没有明显的影响,L-817、818在高剂量时可以抑制HepG2的迁移。

Objective To investigate the effect of somatostatin receptor subtype specific agonists on human hepatocellular carcinoma HepG2 cell line.Methods Effects of SSTR agonists on proliferation、apoptosis and migration of HepG2 were assessed by MTS、the TdT-mediated dUTP nick end labelin assay（TUNEL） and transwell,respectively.Results Compared with the controls,none of four SSTR agonists influenced proliferation or apoptosis of HepG2 in experimental group.However,SSTR5 agonist（L-817,818） showed an obvious inhibitory effect on migration when dosage was 1μg/ml and there was a statistical significance when the dosage reached at 5μg/ml（P0.05）.Conclusion SSTR agonists have no significant effects on proliferation and apoptosis of HepG2.SSTR5 agonist（L-817,818） shows an obvious inhibitory effect on migration when the dosage is increasing.