苦参碱和氧化苦参碱影响戊巴比妥钠催眠作用的实验研究

Effect of Matrine and Oxymatrine on Pentobarbital Sodium Induced Hypnosis

目的研究苦参碱和氧化苦参碱诱导小鼠肝药酶后对戊巴比妥钠催眠作用的影响。方法 ICR雄性小鼠98只随机分为对照组以及苦参碱和氧化苦参碱肝药酶诱导组。对照组给予生理盐水,肝药酶诱导组分别等容积灌胃给予苦参碱(20、40和80mg.kg-1)或氧化苦参碱(20、40和80mg.kg-1)预处理7d,并于末次给药24h后,各组腹腔注射戊巴比妥钠(40mg.kg-1),观察并记录小鼠催眠潜伏期和催眠时间。结果与对照组相比,苦参碱预处理组和氧化苦参碱预处理组均可使戊巴比妥钠对小鼠的催眠时间缩短(P<0.05或0.01);氧化苦参碱预处理组(40和80 mg.kg-1)还可分别缩短戊巴比妥钠对小鼠催眠的潜伏期(P>0.05)。结论苦参碱和氧化苦参碱预处理可诱导小鼠肝药酶而拮抗戊巴比妥钠的催眠作用。

Objective To examine the effect of matrine and oxymatrine on pentobarbital induced hypnosis via enhanced activities of cytochrome P450.Methods A total of 98 ICR male mice were randomly assigned to receive oral administration of physiologic saline（control）,matrine（20,40 or 80mg·kg-1） or oxymatrine（20,40 or 80mg·kg-1） pretreated for 7 days to induce cytochrome P450,and following the 24hr interval after last administration,all animals received an intraperitoneal injection of pentobarbital sodium（40mg·kg-1,Body Weight）.The sleeping time and sleep latency were recorded.Results Pretreatment of matrine and oxymatrine（20,40 and 80mg·kg-1） significantly decreased pentobarbital-induced sleeping time.Pretreatment of oxymatrine（40 and 80mg·kg-1） reduced sleep latency.Conclusion Matrine and oxymatrine can induce cytochrome P450 and antagonize pentobarbital induced hypnosis.