响应面法优化β-葡萄糖苷酶均相水解的工艺条件

Study on Technology Optimization of Homogeneous Hydrolysis of β-glucosidase by Response Surface Methodology

以蒸汽爆破玉米秸秆酶水解液为底物,选取温度、pH和酶用量为自变量,水解液中的葡萄糖含量为响应值,利用Box-Benhnken中心组合试验和响应面(RSM)分析法对β-葡萄糖苷酶均相水解条件进行优化。结果表明:水解温度和酶用量对葡萄糖含量影响显著;β-葡萄糖苷酶均相水解纤维二糖的最佳工艺为:温度52℃,pH4.0,酶用量1 IU/mL。在此条件下,3次验证实验的平均葡萄糖浓度为52.81 mg/mL。

Box-Benhnken central composite design and Response Surface Methodology（RSM） were applied to optimize the hydrolytic conditions from homogeneous hydrolysis of β-glucosidase by taking enzymatic saccharification of steam-exploded corn stover as substrate,and temperature,pH value and β-glucosidase dosage as independent variables,while taking the glucose content in the homogeneously hydrolyzed liquor as dependent variable in the experiment.The results showed that the glucose content was significantly affected by temperature and β-glucosidase dosages.The optimum conditions for homogeneous hydrolysis of cellobiose enzymatic saccharification were concluded as： 52 ℃ as reaction temperature,pH value 4.0,1 IU/mL as β-glucosidase dosage.Under these conditions,the average glucose content reached 52.81mg/mL in the 3 verification tests.