摘要
本研究构建了西伯利亚鲟（Acipenser baeri）扩增片段长度多态性（AFLP）分析体系,分析了DNA提取、双酶切反应、连接反应、预扩增反应、选择性扩增反应和银染等步骤。用7对引物对24尾西伯利亚鲟进行了AFLP分析,每对引物扩增的位点数在24～39之间,共扩增出了234个位点,多态性位点为116个,多态性比例为48.83%,平均Nei氏基因多样性指数H为0.4297,平均Shannon氏指数I为0.1233,个体间平均遗传距离为0.1872,表明西伯利亚鲟养殖群体的遗传多样性程度比较低,同时还构建了西伯利亚鲟的聚类图。
An AFLP（Amplified Fragment Length Polymorphism） analysis system was established including DNA extraction,double enzymes digestion reaction,adapter ligation reaction,pre-amplification and selective amplification reactions,and argent dyeing and the processes were presented for Siberian sturgeon Acipenser baeri.Amplified fragment length polymorphism of DNA（AFLP） is a kind of molecular markers famous for its higher sensitivity and capability.In the study,7 primer pairs were used to amplify 24 Acipenser baeri genomic DNA samples by AFLP method.There are total of 234 loci detected with 24～39 loci per primer pair,in which 116 loci are polymorphic with polymorphisms of 48.83%.The mean Nei＇s genetic diversity index（H） is found 0.4297,the mean Shannon＇s information index（I） 0.1233,showing a relative lower genetic polymorphism.The mean genetic distance among individuals is found 0.1872.Meanwhile,the DNA molecular dendrogram of Acipenser baeri is represented here.
出处
《水产学杂志》
CAS
2011年第1期8-13,共6页
Chinese Journal of Fisheries
基金
公益性行业（农业）科研专项课题“牙鲆性别控制与史氏鲟、黄颡鱼性别标记开发”（200903046-07）
