可溶性CD44v6、VEGF检测在结核性腹水和卵巢癌性腹水鉴别诊断中的价值

Clinical values of detecting soluble CD44v6 and VEGF in differentiate tuberculous ascites and ascites in ovarian can cer

目的探讨可溶性CD44变异性外量子(sCD44v6)及血管内皮生长因子(VEGF)对结核性、卵巢癌性腹水的诊断价值。方法选取39例卵巢癌性腹水、48例结核性腹水采用酶联免疫吸附试验(ELISA)检测腹水中sCD44v6及VEGF的水平,并进行相关性分析。结果卵巢癌性腹水组sCD44v6水平为(102±31)ng/ml,明显高于结核性腹水组(44±15)ng/ml,差异有统计学意义(P<0.01)。卵巢癌性腹水组VEGF水平为(684±210)ng/ml,明显高于结核性腹水组(90±11)ng/ml,差异有统计学意义(P<0.01)。sCD44v6以>60.5 ng/ml为阳性界值,sCD44v6诊断卵巢癌性腹水的敏感性为82.1%(32/39),特异性为87.5%(42/48);VEGF以>101.3 ng/ml为阳性界值,VEGF诊断卵巢癌性腹水的敏感性为92.3%(36/39),特异性为89.9%(43/48)。结论癌性腹水中的CD44v6、VEGF水平明显升高,sCD44v6、VEGF的检测对于结核性、卵巢癌性腹水的鉴别诊断有重要价值。

Objective To investigate the diagnostic value of soluble CD44v6（sCD44v6） and VEGF in the tuberculous ascites and ovarian cancer ascites.Methods Used enzyme linked immunosorbent assay（ELISA） to detect sCD44v6 and VEGF levels in 39 cases of ovarian cancer ascites,and 48 cases of tuberculous ascites.Results sCD44v6 was（102±31 ） ng/ml in ovarian cancer ascites group,which was significantly higher than tuberculous ascites（44±15） ng/ml（P〈0.01 ）. VEGF was（684±210） ng/ml in ovarian cancer ascites,which was significant different from tuberculous ascites（90±11） ng/ ml（P〈0.01）.sCD44v6〉60.5 ng/ml was defined as positive critical values,diagnosis of ovarian cancer ascites by sCD44v6 were as followed,sensitivity was 82.1%（32/39）,specificity was 87.5%（42/48）;VEGF〉101.3 ng/ml were defined as positive critical values,diagnosis of ovarian ascites by VEGF were as followed,sensitivity was 92.3%（36/39）, specificity was 89.9%（43/48）.Conclusion CD44v6 and VEGF in malignant ascites were significantly increased,detection of sCD44v6 and VEGF were of great value in differentiate ascites of tuberculosis and ovarian cancer.