产β-内酰胺酶伤寒杆菌与痢疾杆菌blaTEM耐药基因研究

Study on β-lactamase Producing Salmonella typhi and Shigella Resistance Gene of blaTEM

目的:研究新余市产β-内酰胺酶伤寒杆菌与痢疾杆菌blaTEM-1D型耐药基因流行特征。方法:收集新余新钢中心医院、新余市人民医院、新余市中医院、新余市妇幼保健院2008年1月—2010年6月分离出的伤寒杆菌产酶株6株和痢疾杆菌产酶株13株共19株,用头孢哨噻吩滤纸片法做β-内酰胺酶的定性检测,确定为产酶株后,采用PCR技术扩增质粒blaTEM-1D耐药基因,用Mark标记量出扩增子的大小(bp),统计blaTEM-1D耐药基因阳性率。结果:伤寒杆菌、痢疾杆菌产酶株blaTEM-1D耐药基因阳性率分别为66.7%(4/6)、76.9%(10/13)。结论:blaTEM-1D型耐药基因在本地区各家医院间存在水平传播和医院内垂直传播。

Objective：To study epidemiological characteristics of the β-lactamase producing Salmonella typhi in Xinyu and Shigella blaTEM-1D-type resistance gene.Methods： Isolated enzyme production strains of Salmonella typhi 6 and Shigella 13,a total of 19 strains were collected from the Xinyu Steel Central Hospital,People＇s Hospital of Xinyu,Chinese Medicine Hospital of Xinyu,Maternal and Child Health Hospital of Xinyu from January 2008 to June 2010.Cephalosporins post filter paper method was used to do thiophene β-lactamase qualitative detection.Affter it was defined as enzyme production strains,the lift vector plasmid was amplified by PCR blaTEM-1D resistance gene.Mark was used to measure the amplification the size of sub（bp length）;blaTEM-1D resistance gene-positive rate was kept.Results： Enzyme production strains of Salmonella typhi and Shigella blaTEM-1D resistance gene positive rates were 66.7%（4/6）,76.9%（10/13）.Conclusion： BlaTEM-1D resistance gene has made vertical transmission and horizontal transmission in the local hospitals.