当归治疗大鼠放射性肺损伤的实验研究

Experimental research of Chinese Angelica in rats with radiation-induced lung injury

目的:观察当归对大鼠放射性肺损伤的治疗作用及对相关氧化指标的影响。方法:大鼠行直线加速器照射建立放射性肺损伤模型,以中药当归和地塞米松腹腔内注射,对大鼠肺组织作病理检查,检测肺组织中超氧化物歧化酶(SOD)、过氧化脂质(MDA)及羟脯氨酸(HP)含量的变化。结果:当归组肺组织急性炎症明显轻于单纯照射组;45d和60d局灶性纤维化不明显。肺组织SOD活性当归组照射后明显高于单纯照射组(P<0.01)。肺组织MDA含量照射后当归组明显低于单纯照射组(P<0.01)。肺组织HP含量照射后45d、60d单纯照射组、地塞米松组高于当归组(P<0.05)。结论:当归可提高SOD活性,减轻氧自由基损伤,抑制胶原沉积,保护肺组织,对放射性肺损伤有治疗作用。

Objective：To study the effects and mechanism of Chinese Angelica against radiation-induced lung injury in rats.Methods：Rats in Groups A,B and C were exposed to 6 Mv X-rays at a dose of 20 Gy thoracically.The rats in Groups B and C were treated with angelica and dexamethasone respectively by gastrogavage one time a day just after X-ray exposure.The rats were sacrificed at day 5,15,30,45 and 60 after X-ray exposure.Pathological observation was performed after the lung tissues were treated with HE and Masson staining.The levels of superoxide dismutase（SOD）,malondialdehyde（MDA）and hydroxyproline（HP）in lung tissues were measured simultaneously.Results：The inflammations in Groups B and C were much slighter compared with Group A.Collagenous fibers proliferation was found in the interstitial lung tissues on day 60 in Groups A and C,but not in Group B.SOD activities in lung tissues of rats in Group B began increasing significantly on day 45 and 60 after X-ray exposure compared with Group A（P0.01）.MDA in lung tissues in group B began decreasing significantly on day 60 after X-ray exposure compared with Group A（P0.01）.On day 60 after X-ray exposure,HP levels in lung tissues of Groups A and C were significantly higher than in Group D（P0.05）.Conclusions：Angelica may improve the inflammatory reaction of radiation-induced lung injury and reduce pulmonary fibrosis caused by radiation injury.The mechanism of angelica of anti-inflammation and anti-fibrosis may possibly result from its potent anti-oxidation effects.