热激后冰浴时间与复苏时间对化学转化法转化效率的影响

Effects of the Ice Bath Time after Heat Shock and the Recovery Time on the Transformation Efficiency of Chemical Transformation

[目的]研究化学转化过程中热激后冰浴时间和复苏时间对转化效率的影响,确定简便快速的转化方法。[方法]采用2种缓冲溶液制备感受态,以热激后冰浴时间或复苏时间为变量,研究这2个因素与转化效率的关系。[结果]冰浴2 min,复苏30或40 min达到最佳转化效率;冰浴和复苏时间为0 min时仍能获得相当数量的转化子。[结论]热激后冰浴和复苏时间对转化效率有一定影响,但不是决定性因素。因此,在一般性转化试验中可以省略这2个步骤以节省时间。

[Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the recovery time on the transformation efficacy,and to confirm the simple and quick transformation method.[Method] The competence was prepared with two buffer solutions;with the ice bath time after heat shock and the recovery time as the variables,the relationship between these two factors and transformation efficacy was studied.[Result] The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min;when the ice bath time and the recovery time was 0 min,certain amount of transformants still could be obtained.[Conclusion] The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy,but they were not the decisive factors.Therefore,in the general transformation experiment,these two steps could be omitted to save time.