黄绿木霉菌菌株产几丁质酶发酵条件的研究

Study on the Fermentation Conditions of Trichoderma aureoviride sp. for the Production of Chitinase

[目的]研究黄绿木霉菌(Trichoderma aureoviride sp.)产几丁质酶的最佳发酵条件,为几丁质酶工业提供新酶源。[方法]采用正交试验设计,以DNS法测定酶解后糖含量变化为指标,对发酵条件进行优化。[结果]在以2%胶体几丁质为碳源、2%蛋白胨为氮源、摇床转速为170 r/min时,黄绿木霉菌产几丁质酶的最佳发酵条件组合:培养基的初始pH为5,接种量为8%,瓶装量为20 ml,在28℃下培养6 d。[结论]试验确定了黄绿木霉菌产几丁质酶最佳条件,为其在工业生产中的应用奠定了基础。

[Objective] The paper was to study the best fermentation conditions of Trichoderma aureoviride sp.for the production of chitinase,thus provide new enzyme source for citinase industry.[Method] Using orthogonal experimental design,with the variation of sugar content after enzymatic hydrolysis measured by DNS method as the indicator,the fermentation conditions were optimized.[Result] Taking colloidal chitin as the carbon source and 2% peptone as the nitrogen source with the shaking speed of 170 r/ min,the optimum fermentation conditions of T.aureoviride for the production of chitinase were as follows： initial pH value of medium,5.0;inoculation amount,10%;bottle volume,20 ml;6 d cultivation at 28 ℃.[Conclusion] The optimum conditions for the production of chitinase were confirmed,which provided basis for the utilization of T.aureoviride in the industry.