摘要
[目的]研究大片段DNA提取和纯化的优化方案研究。[方法]在传统SDS提取法的基础上,对大片段DNA的提取和纯化过程进行了改进。[结果]建立了一种优化的DNA提取和纯化的方案,通过该方案能够得到片段大、纯度高的DNA。[结论]为今后大片段DNA的提取和纯化提供了参考。
[Objective] The aim was to study the optimum proposal of big segment DNA extraction and purification.[Method] Based on traditional SDS extraction,the extraction and purification process of big segment DNA were improved.[Result] A optimized scheme of DNA extraction and purification was established,based on this scheme,DNA with big segment and high purity were obtained.[Conclusion] All those provided references for big segment DNA extraction and purification in the future.
出处
《安徽农业科学》
CAS
北大核心
2011年第9期5096-5097,5100,共3页
Journal of Anhui Agricultural Sciences
基金
中国大洋矿产资源研究开发协会资助项目(DYXM-115-02.2.14)
关键词
大片段DNA
提取
纯化
优化方案
Big segment DNA
Extraction
Purification
Optimized scheme
