甘蔗脱毒健康种苗宿根矮化病的PCR检测

Detection of Ratoon Stunting Disease in Virus-free Seedcane of Saccharum officinarum by PCR

[目的]为甘蔗脱毒健康种苗宿根矮化病的检测提供好的方法。[方法]利用PCR技术对甘蔗脱毒健康种苗进行甘蔗宿根矮化病(RSD)病菌的检测研究。[结果]在甘蔗健康种苗生长的前几个不同时期(组培苗增殖、生根、丛苗沙培和单株假植阶段),甘蔗宿根矮化病菌的检测结果均为阴性。对PCR检测灵敏度的研究表明,PCR能够检测到甘蔗宿根矮化病菌液浓度10-3。[结论]PCR检测特异性好、灵敏度高,适合甘蔗脱毒健康种苗生产中的大批量检测。

[Objective] The study was carried out for providing good method to detect ratoon stunting disease（RSD） in virus-free seedcane of Saccharum officinarum.[Method] PCR method was used for the detection of RSD in virus-free seedcane of sugarcane.[Result] During the different growth stages of healthy virus-free seedling including proliferation stage,rooting stage,sand culture stage and temporary planting stage,the RSD detection results were negative.PCR detection sensitivity in the present study showed that PCR could detect RSD pathogen in the solution with bacterium concentration 10-3.[Conclusion] PCR method showed a good specificity and higher sensitivity,so it was suitable for the batch detection in the production of healthy virus-free seedlings of sugarcane.