PCR引物网络与软件设计的一次成功实践

A Successful Practice of the Primer Design Based on Network and some Software

文章基于网络及BLAST,Primer Premer5等辅助软件,对苹果18SrRNA片段、ABP基因全长序列的PCR引物进行设计与探讨,完成了ABP正义表达基因和反义表达基因的扩增引物设计.结果表明,在PCR产物电泳检测的预计大小处有条带出现,测序分析证实所获得片段为目的基因.此次引物的成功设计,为相关基因克隆研究提供了保证,更为今后的PCR引物设计积累了经验.

Based on network and some softwares as：BLAST,Primer Premer5,the study has finished designing ABP gene′s full length sequence and the specific primer of 18SrRNA,especially designing the amplified primer of ABP sense and anti-sense gene.The result indicates that the segments obtained during the test of electrophoresis are confirmative to be the target genes.This successful design of PCR primer provides a strong support to the academic research on relevant gene cloning and also valuable practice for the further design about correlative PCR primer.